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Cloning and Expression
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Stem Cell Culture Media
Expansion Media
Subcutaneous Preadipocyte Medium, 500ml
Subcutaneous Preadipocyte Medium, 500ml from Zen-Bio, Inc.
Be the first to write a review!
Citations:
(44)
Supplier Page
Supplier Page from
Zen-Bio, Inc. for
Subcutaneous Preadipocyte Medium, 500ml
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Company Info
Citations
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Product Specs
Item
Subcutaneous Preadipocyte Medium, 500ml
Company
Zen-Bio, Inc.
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
PM-1
Quantity
500ml
Temperature
4C
Type
MEDIA
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Zen-Bio, Inc.
3920 South Alston Ave
Durham
,
North Carolina
27713
United States
Phone
: (866)234-7673 or (919)547-0692
(919)547-0693
Company Profile
Website:
http://www.zen-bio.com
Citations (44)
(1)
Human Preadipocytes Differentiated under Hypoxia following PCB126 Exposure during Proliferation: Effects on Differentiation, Glucose Uptake and Adipokine Profile
Cells
September 21, 2023
Zeinab El Amine, Jean-François Mauger, Pascal Imbeault
(Durham, NC, USA) and cultured according to the manufacturer’s instructions. Cells suspended in
Zenbio
’s
PM-1
culture media were seeded in culture-treated 96-well plates at a density of at least 40,000 cells/cm2.
(2)
IGFBP2 secretion by mammary adipocytes limits breast cancer invasion
Science Advances
July 14, 2023
James R. W. Conway, Defne D. Dinç, Gautier Follain, Oona Paavolainen, Jasmin Kaivola, Pia Boström, Pauliina Hartiala, Emilia Peuhu, Johanna Ivaska
Scientific), 24-well plates (Cellstar), or μ-Slide 8 well (Ibidi, 80826) in subcutaneous preadipocyte medium (
PM-1
;
ZenBio
) for 2 to 3 days to obtain preadipocytes. For differentiation initiation,
PM-1
was carefully
(3)
Differentiation of human subcutaneous adipocytes and measurement of lipolytic function induced by GIP or LY3437943
STAR Protocols
June 1, 2023
Ajit Regmi, William Roell
proteins" ,, bRecovery™
Cell Culture
Freezing Medium,
Thermo Fisher
,
12648010
cSubcutaneous Preadipocyte Medium,
Zenbio
,
PM-1
cDifferentiation Medium,
Zenbio
,
DM-2
cAdipocyte Medium,
Zenbio
,
AM-1
cDMEM,Gibco,11054-020 cEGM™-2
(4)
Maresin 1 Exerts a Tissue-Specific Regulation of Adipo-Hepato-Myokines in Diet-Induced Obese Mice and Modulates Adipokine Expression in Cultured Human Adipocytes in Basal and Inflammatory Conditions
Biomolecules
May 31, 2023
Martínez-Fernández L, Burgos M, Sáinz N, Laiglesia LM, Arbones-Mainar JM, González-Muniesa P, Moreno-Aliaga MJ.
to Prieto et al. [29]. Briefly, cryopreserved pre-
adipocytes
were cultured in pre-
adipocyte
medium (
PM-1
; DMEM/Ham’s F-12 medium, HEPES, FBS, penicillin, streptomycin, amphotericin B;
Zen-Bio
) until confluence.
(5)
Exosomal miR-1304-3p promotes breast cancer progression in African Americans by activating cancer-associated adipocytes
Nature communications
December 14, 2022
Zhao D, Wu K, Sharma S, Xing F, Wu SY, Tyagi A, Deshpande R, Singh R, Wabitsch M, Mo YY,
et al
.
HCC1806, T47D, were cultured in RPMI 1640 (Gibco) with 10% FBS.
BR-F
was cultured in Preadipocyte Medium (
PM-1
,
Zen-Bio
). SGBS was cultured in 0F (DMEM/F12 + pantothenate +biotin + penicillin/streptomycin) plus
(6)
Docosahexaenoic Acid Counteracts the Hypoxic-Induced Inflammatory and Metabolic Alterations in 3T3-L1 Adipocytes
Nutrients
November 1, 2022
Noura B. Younes, Omnia Ahmed Mohamed, Nasser M. Rizk
alongside the cells from the same company (
Zen-Bio
). The media were: 3T3-L1Preadipocyte Media, Cat#
PM-1
-L-1 (contains 4.5 g/L D-glucose, equals ~25 mM); 3T3-L1Adipocyte Maintenance Media, Cat#
AM-1
-L-1;
3T3-L1
(7)
Adipose microtissue-on-chip: a 3D cell culture platform for differentiation, stimulation, and proteomic analysis of human adipocytes
Lab on a Chip
August 23, 2022
Nina Compera, Scott Atwell, Johannes Wirth, Christine von Törne, Stefanie M. Hauck, Matthias Meier
(NC, USA) and maintained in routine 2D
cell culture
using subcutaneous preadipocyte growth medium (
PM-1
;
ZenBio
) according to the supplier's protocol. hASCs at passages 6–8 were used for the experiments.
(8)
The Combined Partial Knockdown of CBS and MPST Genes Induces Inflammation, Impairs Adipocyte Function-Related Gene Expression and Disrupts Protein Persulfidation in Human Adipocytes
Antioxidants (Basel)
May 31, 2022
Jessica Latorre, Angeles Aroca, José Manuel Fernández-Real, Luis C. Romero, José María Moreno-Navarrete
kg/m2 (Zen-Bio Inc., Research Triangle Park, Durham, NC, USA) were cultured with Preadipocyte Medium (
PM-1
,
Zen-Bio Inc
.) in a humidified incubator at 37 °C with 5% CO2. Twenty-four hours after plating, cells
(9)
Long Noncoding RNA GAS5 Contained in Exosomes Derived from Human Adipose Stem Cells Promotes Repair and Modulates Inflammation in a Chronic Dermal Wound Healing Model
Biology (Basel)
March 11, 2022
Rekha S. Patel, Sabrina Impreso, Ashley Lui, Gitanjali Vidyarthi, Paul Albear, Niketa A. Patel
were purchased from
Zenbio
(catalog #
ASC-F
) and grown to confluency in its media (
Zenbio
, catalog #
PM-1
; also called
stem cell
media as it maintains the stemness of hASC). The hASCs were characterized by
(10)
A microRNA Cluster Controls Fat Cell Differentiation and Adipose Tissue Expansion By Regulating SNCG
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
February 1, 2022
Rodríguez-Barrueco R, Latorre J, Devis-Jáuregui L, Lluch A, Bonifaci N, Llobet FJ, Olivan M, Coll-Iglesias L, Gassner K, Davis ML,
et al
.
purchased (
Zen‐Bio, Inc
.). To induce differentiation, confluent preadipocytes in preadipocyte medium (
PM‐1
) were incubated with
adipocyte
differentiation medium (
DM‐2
) for 7 days, according to manufacturer's
(11)
IL-1β and TNFα Cooperativity in Regulating IL-6 Expression in Adipocytes Depends on CREB Binding and H3K14 Acetylation
Cells
November 19, 2021
Al-Roub A, Al Madhoun A, Akhter N, Thomas R, Miranda L, Jacob T, Al-Ozairi E, Al-Mulla F, Sindhu S, Ahmad R.
SP-F-1
,
OP-F-1
, and
OP-F-3
, respectively). The cells were maintained in preadipocyte growth medium (
PM-1
,
ZenBio
, NC, USA). At 80% confluency, cells were differentiated into
adipocytes
in differentiation medium
(12)
Elevated circulating follistatin associates with an increased risk of type 2 diabetes
Nature communications
November 10, 2021
Wu C, Borné Y, Gao R, López Rodriguez M, Roell WC, Wilson JM, Regmi A, Luan C, Aly DM, Peter A,
et al
.
plates (160,000 cells/cm2) in 100 µL EGM2-MV medium (Zenbio). After 24 h, the medium was replaced with
PM1
(
Zenbio
) for 24 h, followed by initiation of differentiation with
DM2
(
Zenbio
) medium for 6 days. Subsequently,
(13)
Upscaling of pneumatic membrane valves for the integration of 3D cell cultures on chip
Lab on a Chip
June 18, 2021
Nina Compera, Scott Atwell, Johannes Wirth, Bernhard Wolfrum, Matthias Meier
(
StemCell Technologies
, Canada) + 1% penicillin–streptomycin and subcutaneous preadipocyte growth medium (
PM-1
;
ZenBio
, NC, USA), respectively. Prior to use in cell experiments, the chip was coated with 10% Pluronic
(14)
Angiopoietin-like protein 8 differentially regulates ANGPTL3 and ANGPTL4 during postprandial partitioning of fatty acids[S]
Journal of Lipid Research
August 1, 2020
Yan Q. Chen et al.
96-well plates (160,000 cells/cm2 in 100 μl of EGM2-MV media; Zen-Bio). After 24 h, media was replaced by
PM1
(
Zen-Bio
) followed by replacement with
DM2
(
Zen-Bio
) media. Fresh
DM2
was added on the third day followed
(15)
PPARγ and PPARα synergize to induce robust browning of white fat in vivo
Molecular metabolism
June 1, 2020
Kroon T, Harms M, Maurer S, Bonnet L, Alexandersson I, Lindblom A, Ahnmark A, Nilsson D, Gennemark P, O'Mahony G,
et al
.
previously described [35]. Briefly, isolated cells were seeded and cultured in proliferation medium (
Zenbio
,
PM-1
) containing 3% FBS Gold (PAA, A15-104), 1% penicillin/streptomycin and 1 nM bFGF (
Sigma
,
F0291
). After
(16)
Chronic Intermittent Hypoxia Triggers a Senescence-like Phenotype in Human White Preadipocytes
Scientific reports
April 22, 2020
Polonis K, Becari C, Chahal CAA, Zhang Y, Allen AM, Kellogg TA, Somers VK, Singh P.
abdominal adipose tissue
of healthy individuals (ZenBio Inc., North Carolina). HWPs were grown in complete medium (
PM-1
,
ZenBio
, Inc., North Carolina) and used for experiments in 3-5 passage with a 50–60% confluence. A previously
(17)
Breast Adipocyte Co-culture Increases the Expression of Pro-angiogenic Factors in Macrophages
Frontiers in Oncology
April 7, 2020
Nalini V. S. Yadav, Arthur Barcikowski, Yuko Uehana, Aaron T. Jacobs, Linda Connelly
commercially obtained (
ZenBio
, Research Triangle Park, NC, USA) and expanded in Preadipocyte Medium (
PM-1
). All cells were maintained at 37°C in humidified incubator containing 5% CO2. THP-1 were differentiated
(18)
Melatonin Modulation of Radiation and Chemotherapeutics-induced Changes on Differentiation of Breast Fibroblasts
International Journal of Molecular Sciences
August 13, 2019
Alicia González-González, Enrique García Nieto, Alicia González, Cristina Sánchez-Fernández, Carolina Alonso-González, Javier Menéndez-Menéndez, José Gómez-Arozamena, Samuel Cos, Carlos Martínez-Campa
USA). They were maintained as monolayer cultures in 58,2 cm2 plastic culture plates in Preadipocyte Medium (
PM-1
) (
ZenBio
, Inc, Research Triangle Park NC, USA) supplemented with penicillin (20 units/mL) and streptomycin
(19)
Identification of a mesenchymal progenitor cell hierarchy in adipose tissue
Science (New York, N.Y.)
April 26, 2019
Merrick D, Sakers A, Irgebay Z, Okada C, Calvert C, Morley MP, Percec I, Seale P.
purified; plated on Corning CellBind 384-, 96-, or 48-well plates (Sigma-Aldrich); and first cultured in
PM-1
medium (
Zenbio
catalog no.
PM-1
) supplemented with 1 nM recombinant fibroblast growth factor 2 (FGF2)
(20)
Stabilization of lncRNA GAS5 by a Small Molecule and Its Implications in Diabetic Adipocytes
Cell chemical biology
March 21, 2019
Shi Y, Parag S, Patel R, Lui A, Murr M, Cai J, Patel NA.
purchased from Zenbio™. The cells were cultured as follows. ASC were passaged with preadipocyte medium (
PM-1
; DMEM/Ham’s F-12 medium, HEPES, FBS, penicillin, streptomycin, amphotericin B;
ZenBio
™) and then plated
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Subcutaneous Preadipocyte Medium, 500ml from Zen-Bio, Inc.
Supplier Page
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Pricing Info
Product Specs
Company Info
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